Unlocking ABA`s role in rice cold tolerance: insights from Zhonghua 11 and Kasalath

Wenyu Li, Xin Lou, Zhijun Wang, Di Zhang, Lingling Li, Xiaoping Ding, Gongye Cheng, Weiying Nie, Zhilin Li, Jianghui Yu, Jiwai He, Nenghui Ye, Dingyang Yuan, Meijuan Duan & Citao Liu

Theoretical and Applied Genetics; January 3 2025; vol.138; article 16

Key message

Unraveling key ABA pathways, including OsWRKY71-OsABA8ox1 and OsbZIP73-OsNCED5, provides valuable insights for improving cold tolerance in rice breeding for cold-prone regions.

Abstract

Cold stress limits rice (Oryza sativa L.) production in cooler climates. This study uncovers how abscisic acid (ABA) signaling enhances cold tolerance in the rice variety Zhonghua 11 (ZH11) compared to the cold-sensitive Kasalath. Under cold stress, ZH11 rapidly accumulates ABA through efficient regulation of key genes. The transcription factor OsWRKY71^ZH11 represses the ABA catabolism gene OsABA8ox1 during early stress, enabling quick ABA accumulation. Additionally, OsbZIP73 regulates the ABA synthesis gene OsNCED5 to maintain ABA balance during prolonged stress. Transgenic ZH11 plants overexpressing OsWRKY71^ZH11 exhibited enhanced cold tolerance, while overexpression of OsWRKY71^Ka did not confer benefits. Haplotype analysis linked allelic variations in OsWRKY71 and OsNCED5 to differences in cold tolerance. Our findings highlight critical ABA signaling pathways that enhance cold tolerance in rice. Targeting these pathways offers promising strategies for breeding cold-resistant rice varieties, improving resilience in cold-prone regions.

See https://link.springer.com/article/10.1007/s00122-024-04810-x