CRISPR/Cas9 mediated knockout of MeSSI enhances resistant starch content without compromising yield in cassava
Friday, 05-06-2026 | 07:51
Enhancing resistant starch (RS) content in cassava is vital for developing nutritionally improved, functional food crops. In this study, targeted mutagenesis of the MeSSI gene via CRISPR/Cas9 was conducted to investigate its role in starch biosynthesis and RS accumulation. MeSSI knockout lines exhibited a 6.74-fold increase in RS content and a 16.42% elevation in amylose levels compared to the wild-type, without compromising total starch content or root yield.
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CRISPR/Cas9 mediated knockout of MeSSI enhances resistant starch content without compromising yield in cassava
CRISPR/Cas9 mediated knockout of MeSSI enhances resistant starch content without compromising yield in cassava
Enhancing resistant starch (RS) content in cassava is vital for developing nutritionally improved, functional food crops. In this study, targeted mutagenesis of the MeSSI gene via CRISPR/Cas9 was conducted to investigate its role in starch biosynthesis and RS accumulation. MeSSI knockout lines exhibited a 6.74-fold increase in RS content and a 16.42% elevation in amylose levels compared to the wild-type, without compromising total starch content or root yield.
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