MaMAPK3-MaICE1-MaPOD P7 pathway, a positive regulator of cold tolerance in banana

Jie Gao, Tongxin Dou, Weidi He, Ou Sheng, Fangcheng Bi, Guiming Deng, Huijun Gao, Tao Dong, Chunyu Li, Sheng Zhang, Ganjun Yi, Chunhua Hu & Qiaosong Yang

BMC Plant Biology volume 21, Article number: 97 (2021)

Background

Banana is a tropical fruit with a high economic impact worldwide. Cold stress greatly affects the development and production of banana.

Results

In the present study, we investigated the functions of MaMAPK3 and MaICE1 involved in cold tolerance of banana. The effect of RNAi of MaMAPK3 on Dajiao (Musa spp. ‘Dajiao’; ABB Group) cold tolerance was evaluated. The leaves of the MaMAPK3 RNAi transgenic plants showed wilting and severe necrotic symptoms, while the wide-type (WT) plants remained normal after cold exposure. RNAi of MaMAPK3 significantly changed the expressions of the cold-responsive genes, and the oxidoreductase activity was significantly changed in WT plants, while no changes in transgenic plants were observed. MaICE1 interacted with MaMAPK3, and the expression level of MaICE1 was significantly decreased in MaMAPK3 RNAi transgenic plants. Over-expression of MaICE1 in Cavendish banana (Musa spp. AAA group) indicated that the cold resistance of transgenic plants was superior to that of the WT plants. The POD P7 gene was significantly up-regulated in MaICE1-overexpressing transgenic plants compared with WT plants, and the POD P7 was proved to interact with MaICE1.

Conclusions

Taken together, our work provided new and solid evidence that MaMAPK3-MaICE1-MaPOD P7 pathway positively improved the cold tolerance in monocotyledon banana, shedding light on molecular breeding for the cold-tolerant banana or other agricultural species.

See: https://bmcplantbiol.biomedcentral.com/articles/10.1186/s12870-021-02868-z

Figure 1:

(A) Phenotypes of transgenic ‘Dajiao’ plants (MRi-15 and MRi-22) and wild type (WT) subjected to cold treatment (10 °C for 5 and 7 days), followed by recovery at the normal growth temperature for 2 days. (B) MDA content in WT and transgenic Dajiao (RNAi-22 and RNAi-15) before and after chilling treatment. Data represent the means ± SE of at least three replicates. Letters indicate significant differences from 0 h chilling treatment according to the Student–Newman–Keuls test (P < 0.05). (RE means recovery for 2 days)