Evolutionary signatures of the erosion of sexual reproduction genes in domesticated cassava (Manihot esculenta)

Evan M Long, Michelle C Stitzer, Brandon Monier, Aimee J Schulz, Maria Cinta Romay, Kelly R Robbins, Edward S Buckler

G3 (Bethesda); 2025 Feb 5; 15(2):jkae282. doi: 10.1093/g3journal/jkae282.

Abstract

Centuries of clonal propagation in cassava (Manihot esculenta) have reduced sexual recombination, leading to the accumulation of deleterious mutations. This has resulted in both inbreeding depression affecting yield and a significant decrease in reproductive performance, creating hurdles for contemporary breeding programs. Cassava is a member of the Euphorbiaceae family, including notable species such as rubber tree (Hevea brasiliensis) and poinsettia (Euphorbia pulcherrima). Expanding upon preliminary draft genomes, we annotated 7 long-read genome assemblies and aligned a total of 52 genomes, to analyze selection across the genome and the phylogeny. Through this comparative genomic approach, we identified 48 genes under relaxed selection in cassava. Notably, we discovered an overrepresentation of floral expressed genes, especially focused at 6 pollen-related genes. Our results indicate that domestication and a transition to clonal propagation have reduced selection pressures on sexually reproductive functions in cassava leading to an accumulation of mutations in pollen-related genes. This relaxed selection and the genome-wide deleterious mutations responsible for inbreeding depression are potential targets for improving cassava breeding, where the generation of new varieties relies on recombining favorable alleles through sexual reproduction.

See https://pubmed.ncbi.nlm.nih.gov/39673428/

Figure 1. Assembly quality statistics. Assembly size and N50 are shown for long and short-read assemblies we produced, as well as the public assemblies used in this study (a). BUSCO scores are plotted with species’ text color matching sources (b).