CRISPR/Cas9-mediated genome editing in rice cv. IPB3S results in a semi-dwarf phenotypic mutant

NURHAYATI, SINTHO WAHYUNING ARDIE, TRI JOKO SANTOSO, SUDARSONO

B I O D I V E R S I T A S Volume 22, Number 9, September2021 Pages: 3792-3800

Abstract

Nurhayati,  Ardie  SW,  Santoso  TJ,Sudarsono.  2021. CRISPR/Cas9-mediated  genome  editing  in  rice  cv. IPB3Sresults  in a semi-dwarf  phenotypic  mutant.Biodiversitas  22:3792-3800.IPB3Sis Indonesian  lowland  rice and  high-yielding cultivar.  However, plant height posture makes itprone tolodging whichcould reduce the yield. This study aimed to edit the GA20Ox2gene by introducing CRISPR/Cas9 GA20Ox2construct  into IPB3Sand  developing  the  semidwarf  rice  mutants.  Immature  embryo  explants  of IPB3Swere used for thetransformation process mediated by EHA105 strain of Agrobacterium tumefacienscarrying pC1300-Cas9/GA20Ox2and by changing the regeneration medium composition. PCR analysis showed that rice cv. IPB3Sgenetic transformation gained putative mutant T0  lines carrying  the hptgene  (growthefficiency  was 47.9%,  while  transformation  efficiency  was  19.3%).  Using  the  developed regeneration  medium,  we  have  obtained  24putative rice  cv. IPB3ST0 mutant lines  carrying hpt.  The  best  medium  for  regenerating IPB3S  was  the Amedium  (regeneration efficiency  73.3%). IPB  8  and  IPB  14  were  potential  to  evaluate  in  the  next  generation.  The shortest plant height for the T1 generation was observed inthe IPB 8-3mutant.

See: https://www.smujo.id/biodiv/article/view/9132/5114