Biosynthesis of Scopoletin in Sweet Potato Confers Resistance against Fusarium oxysporum

Jing Wang, Peitao Chen, Tengfei Zhao, Xianhui Huang, Jikai Zong, Qingqing Luo, Chao Peng, Xiaoyan Wu, Fei Qiu, Dongchao Zhao, Lien Xiang, Yan Zhang, Chunxian Yang, Fangyuan Zhang, Zhihua Liao, Yufan Fu, Junlan Zeng

J Agric Food Chem.; 2024 Apr 10; 72(14):7749-7764. doi: 10.1021/acs.jafc.3c09389. 

Abstract

Fusarium wilt is a severe fungal disease caused by Fusarium oxysporum in sweet potato. We conducted transcriptome analysis to explore the resistance mechanism of sweet potato against F. oxysporum. Our findings highlighted the role of scopoletin, a hydroxycoumarin, in enhancing resistance. In vitro experiments confirmed that scopoletin and umbelliferone had inhibitory effects on the F. oxysporum growth. We identified hydroxycoumarin synthase genes IbF6'H2 and IbCOSY that are responsible for scopoletin production in sweet potatoes. The co-overexpression of IbF6'H2 and IbCOSY in tobacco plants produced the highest scopoletin levels and disease resistance. This study provides insights into the molecular basis of sweet potato defense against Fusarium wilt and identifies valuable genes for breeding wilt-resistant cultivars.

See https://pubmed.ncbi.nlm.nih.gov/38537104/