A key ABA biosynthetic gene OsNCED3 is a positive regulator in resistance to Nilaparvata lugens in Oryza sativa

Jitong Li, Hao Liu, Xinyi Lv, Wenjuan Wang, Xinyan Liang, Lin Chen, Yiping Wang, Jinglan Liu

Front Plant Sci.; 2024 Jun 26: 15:1359315. doi: 10.3389/fpls.2024.1359315.

Abstract

The gene encoding 9-cis-epoxycarotenoid dioxygenase 3 (NCED3) functions in abscisic acid (ABA) biosynthesis, plant growth and development, and tolerance to adverse temperatures, drought and saline conditions. In this study, three rice lines were used to explore the function of OsNCED3, these included an OsNCED3-overexpressing line (OsNCED3-OE), a knockdown line (osnced3-RNAi) and wild-type rice (WT). These rice lines were infested with the brown plant hopper (BPH; Nilaparvata lugens) and examined for physiological and biochemical changes, hormone content, and defense gene expression. The results showed that OsNCED3 activated rice defense mechanisms, which led to an increased defense enzyme activity of superoxide dismutase, peroxidase, and polyphenol oxidase. The overexpression of OsNCED3 decreased the number of planthoppers and reduced oviposition and BPH hatching rates. Furthermore, the overexpression of OsNCED3 increased the concentrations of jasmonic acid, jasmonyl-isoleucine and ABA relative to WT rice and the osnced3-RNAi line. These results indicate that OsNCED3 improved the stress tolerance in rice and support a role for both jasmonates and ABA as defense compounds in the rice-BPH interaction.

See https://pubmed.ncbi.nlm.nih.gov/38988632/

Figure 1: Bioinformatics analysis of OsNCED3 gene in rice. (A) comparison of deduced protein sequence encoded by OsNCED3. Black part represents highly conserved residues; red represents conservative substitution; light blue represents semi conservative substitution. OsNCED3 (Oryza sativa); ObNCED1 (Oryza brachyantha); SbNCED1 (Sorghum bicolor); ZmNCED1 (Zea mays); BdNCED3 (Brachypodium distachyon); SiNCED1 (Setaria italica). (B) phylogenetic tree of amino acid sequence of OsNCED gene from different sources; OsNCED3 (Oryza sativa); ObNCED1 (Oryza brachyantha); SbNCED1 (Sorghum bicolor); ZmNCED1 (Zea mays); BdNCED3 (Brachypodium distachyon); SiNCED1 (Setaria italica); RsNCED3 (Raphanus sativus); PpNCED1 (Prunus persica); RcNCED1 (Rosa chinensis); CrNCED3 (Capsella rubella); AtNCED3 (Arabidopsis thaliana); BnNCED3 (Brassica napus); BoNCED3 (Brassica oleracea); VvNCED1 (Vitis vinifera); NnNCED1 (Nelumbo nucifera); CmNCED3 (Cucumis melo); PmNCED1 (Prunus mume); CpNCED3 (Cucurbita pepo); MdNCED2 (Malus domestica); CsNCED3 (Cucumis sativus); HaNCED3 (Helianthus annuus); VrNCED1 (Vigna radiata); PsNCED3 (Papaver somniferum); NaNCED1 (Nicotiana attenuata); ZjNCED1 (Ziziphus jujuba); TcNCED3 (Theobroma cacao); MeNCED5 (Manihot esculenta); GsNCED1 (Glycine soja); CcNCED1 (Cajanus cajan); SoNCED1 (Spinacia oleracea). (C) Predicted 3D structure of OsNCED3 using PyMOL software. (D) interacting proteins of OsNCED3 using the STRING database.