Unmasking Tomato Spotted Wilt Virus in Peanuts: From Nucleocapsid Protein Sequence and Localization to Practical Management in Alabama

Abdelaal H A Shehata, Claire Cooke, Wilson Clark, Michael A Mayfield, Livleen Kaur, Alexandra Rios, Katherine B Burch, Henry Jordan, Alana Jacobson, Scott H Graham, Amanda Strayer-Scherer, Kathleen M Martin

Phytopathology; 2026 Mar; 116(3):473-484. doi: 10.1094/PHYTO-06-25-0204-R

Abstract

In 2020, an evaluation of the basal level of incidence of tomato spotted wilt (TSW) in peanut was initiated in Alabama. This was done to understand the viral sequence divergence of tomato spotted wilt virus (TSWV) in a heavily managed system. From 2021 to 2023, 172 leaf samples were collected from peanut plants exhibiting symptoms of TSW from Brewton, Fairhope, and Headland, AL, to investigate genetic changes. Additionally, four thrips populations were collected in 2022 and 2023 from Fairhope and Headland, AL. The nucleocapsid protein of TSWV was sequenced from both leaf samples and thrips. A total of 175 nucleocapsids were sequenced, and their amino acid sequences were aligned to identify three conserved mutations compared with TSWV-MT2 (X61799.1) and seven conserved mutations when aligned against TSWV-BR-01 (D00645.1). Interestingly, only one conserved mutation was found in the thrips sequences obtained via Sanger sequencing compared with MT2, and no mutations were detected when aligned with BR-01. To identify if mutations caused a phenotype that could be measured, eight nucleocapsids carrying the three conserved mutations, in addition to unique changes, were selected for localization in Nicotiana benthamiana. The localization patterns of these proteins were grouped into three phenotypes based on the observed protein aggregation speed. Small-plot trials assessed TSWV mutations and incidence in relation to planting date, insecticide use, and cultivar. Based on these data, although management strategies are effective at keeping levels of TSWV manageable, the virus can diversify its sequence, which causes changes in the expected phenotype of the nucleocapsid. [Formula: see text] Copyright © 2026 The Author(s). This is an open access article distributed under the CC BY-NC-ND 4.0 International license.

See: https://pubmed.ncbi.nlm.nih.gov/41264774/

Fig. 1.Maximum-likelihood phylogenetic trees of tomato spotted wilt virus nucleocapsid (N) proteins. A, Full tree constructed from all 167 N sequences from peanut in Alabama from 2021 to 2023 and GenBank (including Georgia, North Carolina, Hawaii, Italy, South Korea, Japan, China, and Australia). Samples highlighted in red indicate N phenotypes selected for localization in plant cells, including the MT2 strain as a reference. B, Simplified tree showing a subset of representative sequences to improve readability while retaining the main clustering patterns.