Development of Drought-Tolerant Transgenic Wheat: Achievements and Limitations.

Update date: 16 July 2019

Khan S, Anwar S, Yu S, Sun M, Yang Z, Gao ZQ.

Int J Mol Sci. 2019 Jul 8; 20(13). pii: E3350. doi: 10.3390/ijms20133350.

Abstract

Crop yield improvement is necessary to keep pace with increasing demand for food. Due to climatic variability, the incidence of drought stress at crop growth stages is becoming a major hindering factor to yield improvement. New techniques are required to increase drought tolerancealong with improved yield. Genetic modification for increasing drought tolerance is highly desirable, and genetic engineering for droughttolerance requires the expression of certain stress-related genes. Genes have been identified which confer drought tolerance and improve plant growth and survival in transgenic wheat. However, less research has been conducted for the development of transgenic wheat as compared to rice, maize, and other staple food. Furthermore, enhanced tolerance to drought without any yield penalty is a major task of genetic engineering. In this review, we have focused on the progress in the development of transgenic wheat cultivars for improving droughttolerance and discussed the physiological mechanisms and testing of their tolerance in response to inserted genes under control or field conditions

See https://www.ncbi.nlm.nih.gov/pubmed/31288392

Table 1. Improving drought tolerance of wheat through engineering gene.

Transgene	Transgenic Recipient	Source	Improved Traits	References
TaWRKY2	Fielder, a spring Triticum aestivum cultivar	Xifeng20, a drought tolerant wheat	Higher survival rate, proline, soluble sugar and chlorophyll.	[70]
calcineurin B-like protein (CBL)-interacting protein kinase CIPK23	Fielder, a Triticum aestivum cultivar	Triticum aestivumcultivar Xiaobaimai	Higher survival rate, increased osmolytes, induction of stomatal closure, enhanced ABA sensitivity.	[91]
aldose reductase gene MsALR	CY-45, a spring Triticum aestivum cultivar	Alfalfa	Higher detoxification activity for the aldehyde substrate; higher biomass and seed weight.	[96]
HVA1	Hi-Line, a spring Triticum aestivum cultivar	Barley	Improved biomass and water use efficiency.	[48]
HVA1	Hi-Line, a spring Triticum aestivum cultivar	Barley	Higher plant height, total biomass and grain yield.	[49]
Mannitol-1-phosphate dehydrogenase mtlD	Bobwhite, Triticum aestivum cultivar	Escherichia coli	Improved biomass, mannitol accumulation.	[18]
betA encoding choline dehydrogenase	Jinan 17, Triticum aestivum cultivar	Escherichia coli	Accumulation of glycinebetaine.	[32]
Betaine aldehyde dehydrogenase, BADH	Triticum aestivum	Atriplex hortensis	Higher BADH activity, show normal growth.	[35]
Betaine aldehyde dehydrogenase, BADH	Line (T6), from Shi4185 line	Atriplex hortensis	Accumulation of glycinebetaine.	[31]
Betaine aldehyde dehydrogenase, BADH	Line (T6), from Shi4185 line	Atriplex hortensis	Decreased PSII photoinhibition.	[36]
GmDREB	Lumai22, Triticum aestivum cultivar	Glycine max cultivar Jinong27	Improved drought tolerance with more leaves, roots and high soluble sugar contents.	[17]
Δ¹-pyrroline-5 carboxylate synthetase, P5CS	CD200126, Triticum aestivum cultivar	Vigna aconitifolia	Proline biosynthesis.	[19]
Δ¹-pyrroline-5 carboxylate synthetase, P5cs	Triticum aestivum	Triticum aestivum	Proline accumulation.	[28]
DREB1A	bread wheat	Arabidopsis thaliana	More branched root phenotype higher total number of heads, enhance drought tolerance.	[62]
sedoheptulose-1, 7-bisphosphatase SBPase	Line (T2) from cultivar Cadenza	Brachypodium distachyon	SBPase promoter fully drive the GUS expression.	[97]
HaHB4	cv. Cadenza	Sunflower	Increased yield and water use efficiency.	[16]
AtWRKY30	Sakha-61 genotype, Triticum aestivum	Arabidopsis thaliana	Higher biomass, photosynthesis, relative water content, prolines, soluble proteins, soluble sugars, and antioxidant enzymes activities.	[98]
AtHDG11	Chinese Spring, Triticum aestivum	Arabidopsis thaliana	More yield, higher proline content and photosynthesis, lower stomatal density, lower water loss rate, and increased activities of catalase and superoxide dismutase.	[60]
cold shock protein gene SeCspA	cultivar KN199, winter wheat	Escherichia coli	Higher proline, grain weight and grain yield, less reduction in chlorophyll, low MDA content.	[44]
ferritin gene, TaFER-5B	Jimai5265, wheat cultivar	wheat cultivar, TAM107	Improved leaf iron content and ROS, enhanced drought and temperature tolerance.	[51]
phosphoenolpyruvate carboxylase kinase-related kinase gene, TaPEPKR2	Liaochun10, wheat cultivar	wheat cultivar, TAM107	Enhanced drought tolerance, higher root length.	[89]
TaSHN1	Triticum aestivum cultivar Gladius	Australian drought tolerant genotype RAC875	Lower stomatal density and leaf water loss, and improved recovery after severe drought.	[58]
TaNF-YB4	Triticum aestivum cultivar Gladius	Triticum aestivumcultivar RAC875	More spikes.	[95]
DREB/CBF gene TaRAP2.1Lmut	Triticum aestivum cultivar Gladius	Triticum aestivumcultivar RAC785	Enhanced ability to survive frost and drought.	[99]
OTS1, overly tolerant to salt-1	Triticum aestivumGamtoos-R	Arabidopsis thaliana	Delayed senescence, higher relative water content, photosynthesis and antioxidants.	[86]
TaNAC69	Triticum aestivum cultivar Bobwhite	Triticum aestivum	More root biomass, longer roots.	[77]
TabZIP2	Triticum aestivum cultivar Gladius	Triticum aestivumcultivar RAC875	Fewer spikes and seeds, increased single seed weight.	[82]
DREB	Triticum aestivum cultivar Bobwhite	Triticum durum L. cultivar Langdon	Improved survival, slow growth, delayed flowering, less grain yield.	[100]
DREB	Triticum aestivum cultivar 8901, 5–98, 99–92, Baofeng 104	Arabidopsis thaliana	Still green after 15 d withholding water, high proline contents.	[101]
PEPC	Triticum aestivum cultivar Zhoumai19	Maize	Higher proline, soluble sugar and water use efficiency.	[53]
CspA and CspB	Triticum aestivum cultivar KN199	Escherichia coli	Lower water loss rate and MDA content, higher chlorophyll, proline and yield.	[44]

Development of Drought-Tolerant Transgenic Wheat: Achievements and Limitations.

Abstract

Institute of Agricultural Sciences For Southern Vietnam Address: 121 Nguyen Binh Khiem, Tan Đinh Ward, HCM City, Vietnam Tel: +84.8. 38291746 – 38228371 Website : http://iasvn.org - Email: iasvn@vnn.vn

Institute of Agricultural Sciences For Southern Vietnam
Address: 121 Nguyen Binh Khiem, Tan Đinh Ward, HCM City, Vietnam
Tel: +84.8. 38291746 – 38228371
Website : http://iasvn.org - Email: iasvn@vnn.vn