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Improving gene editing of CRISPR/Cas9 using the callus-specific promoter pYCE1 in cassava (Manihot esculenta Crantz)
Improving gene editing of CRISPR/Cas9 using the callus-specific promoter pYCE1 in cassava (Manihot esculenta Crantz)
Friday, 09-01-2026 | 08:12
Previous studies have demonstrated that an appropriate promoter can drive Cas9 transcription in the CRISPR/Cas9 system, which improves the efficiency of gene editing. Here, we identified and characterized callus-specific promoters to enhance gene editing efficiency in cassava. From the transcriptome data of 11 cassava tissues, the gene named YCE1 was identified to exhibit callus-specific expression. Its promoter (pYCE1) could efficiently and specifically drive EGFP transcription in callus tissues.
A robust 1K-core marker set for wild germplasm management and targeted pre-breeding of rice: development and applications
A robust 1K-core marker set for wild germplasm management and targeted pre-breeding of rice: development and applications
Single-cell transcriptomics reveal how root tissues adapt to soil stress
Single-cell transcriptomics reveal how root tissues adapt to soil stress
A genome-wide association analysis identifies a key candidate gene controlling plant growth habit in chickpea
A genome-wide association analysis identifies a key candidate gene controlling plant growth habit in chickpea
Ey-1 encodes a DEDDh exonuclease in eggplant (Solanum melongena), providing a novel pathway for begomovirus resistance
Ey-1 encodes a DEDDh exonuclease in eggplant (Solanum melongena), providing a novel pathway for begomovirus resistance
A natural variant of an MYC2 gene in soybean contributes to resistance against the common cutworm
A natural variant of an MYC2 gene in soybean contributes to resistance against the common cutworm
Modern Japanese rice cultivars often carry a nonautonomous retrotransposon-insertion mutation at the pathogenesis-related 1b protein gene locus causing reduced resistance to Pyricularia oryzae
Modern Japanese rice cultivars often carry a nonautonomous retrotransposon-insertion mutation at the pathogenesis-related 1b protein gene locus causing reduced resistance to Pyricularia oryzae
Characterization of CXE genes in pineapple and their aroma-related expression during fruit ripening
Characterization of CXE genes in pineapple and their aroma-related expression during fruit ripening
Potential yield and food provisioning gains from rebuilding the world’s coral reef fish stocks
Potential yield and food provisioning gains from rebuilding the world’s coral reef fish stocks
JA Signaling Inhibitor JAZ Is Involved in Regulation of AM Symbiosis with Cassava, Including Symbiosis Establishment and Cassava Growth
JA Signaling Inhibitor JAZ Is Involved in Regulation of AM Symbiosis with Cassava, Including Symbiosis Establishment and Cassava Growth
Investigating cis-regulatory elements and gene expression in multiple tomato varieties using interpretable deep learning
Investigating cis-regulatory elements and gene expression in multiple tomato varieties using interpretable deep learning
A major qualitative trait locus increases antioxidant activity in testaless peanut seeds
A major qualitative trait locus increases antioxidant activity in testaless peanut seeds

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