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Precise integration of large DNA sequences in plant genomes using PrimeRoot editors
Precise integration of large DNA sequences in plant genomes using PrimeRoot editors

A technique for chromosomal insertion of large DNA segments is much needed in plant breeding and synthetic biology to facilitate the introduction of desired agronomic traits and signaling and metabolic pathways. Here we describe PrimeRoot, a genome editing approach to generate targeted precise large DNA insertions in plants. Third-generation PrimeRoot editors employ optimized prime editing guide RNA designs

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