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Overcoming polyploidy pitfalls: a user guide for effective SNP conversion into KASP markers in wheat
Overcoming polyploidy pitfalls: a user guide for effective SNP conversion into KASP markers in wheat

Kompetitive allele-specific PCR (KASP) markers are commonly used in marker-assisted commercial plant breeding due to their cost-effectiveness and throughput for high sample volumes. However, conversion of trait-linked SNP markers from array-based SNP detection technologies into KASP markers is particularly challenging in polyploid crop species, due to the presence of highly similar homeologous and paralogous genome sequences.

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