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CRISPR-Cereal: a guide RNA design tool integrating regulome and genomic variation for wheat, maize and rice
CRISPR-Cereal: a guide RNA design tool integrating regulome and genomic variation for wheat, maize and rice
Wednesday, 18-08-2021 | 08:19

The clustered regularly interspaced short palindromic repeat (CRISPR)-associated protein (Cas) genome editing system (CRISPR-Cas) is revolutionizing agriculture. In this system, a guide sequence that matches to a particular genomic DNA is placed in front of a synthetic RNA that consists of a scaffold sequence necessary for Cas-binding to form a guide RNA (gRNA). gRNA/Cas complex binds to the target DNA that contains a protospacer adjacent motif (PAM) via base-paring and generates a double-strand break (DSB) by Cas protein.

De novo assembly, annotation, and comparative analysis of 26 diverse maize genomes
De novo assembly, annotation, and comparative analysis of 26 diverse maize genomes
A Simple Heat Treatment Increases SpCas9-Mediated Mutation Efficiency in Arabidopsis
A Simple Heat Treatment Increases SpCas9-Mediated Mutation Efficiency in Arabidopsis
Integrated strategies for durable rice blast resistance in sub-Saharan Africa
Integrated strategies for durable rice blast resistance in sub-Saharan Africa
The evolution of parasitism from mutualism in wasps pollinating the fig, Ficus microcarpa, in Yunnan Province, China
The evolution of parasitism from mutualism in wasps pollinating the fig, Ficus microcarpa, in Yunnan Province, China
Genetic mapping reveals BjRf as a candidate gene controlling fertility restoration of the oxa CMS in Brassica juncea
Genetic mapping reveals BjRf as a candidate gene controlling fertility restoration of the oxa CMS in Brassica juncea
Proteome characterization of two contrasting soybean genotypes in response to different phosphorus treatments
Proteome characterization of two contrasting soybean genotypes in response to different phosphorus treatments
Identification of glutathione transferase gene associated with partial resistance to Sclerotinia stem rot of soybean using genome-wide association and linkage mapping
Identification of glutathione transferase gene associated with partial resistance to Sclerotinia stem rot of soybean using genome-wide association and linkage mapping
The Welwitschia genome reveals a unique biology underpinning extreme longevity in deserts
The Welwitschia genome reveals a unique biology underpinning extreme longevity in deserts
Genetic architecture of root and shoot ionomes in rice (Oryza sativa L.)
Genetic architecture of root and shoot ionomes in rice (Oryza sativa L.)
A bean common mosaic virus-resistance gene in the soybean variant V94-5152 was mapped to the Rsv4 locus conferring resistance to soybean mosaic virus
A bean common mosaic virus-resistance gene in the soybean variant V94-5152 was mapped to the Rsv4 locus conferring resistance to soybean mosaic virus
Using probabilistic genotypes in linkage analysis of polyploids
Using probabilistic genotypes in linkage analysis of polyploids

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