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CRISPR/Cas9-induced DNA damage enriches for mutations in a p53-linked interactome: implications for CRISPR-based therapies.
CRISPR/Cas9-induced DNA damage enriches for mutations in a p53-linked interactome: implications for CRISPR-based therapies.
Saturday, 04-12-2021 | 04:14

Inactivating p53 mutations are the most abundant genetic alterations found in cancer. Here we show that CRISPR/Cas9-induced double-stranded DNA breaks enrich for cells deficient in p53 as well as in genes of a core CRISPR-p53 tumor suppressor interactome. Such enrichment could predispose to cancer development and thus pose a challenge for clinical CRISPR use. Transient p53 inhibition could suppress the enrichment of cells with these mutations. The level of DNA damage response induced by an sgRNA influenced the enrichment of p53 deficient cells and could be a relevant parameter in sgRNA design to limit cellular enrichment.

Plant–environment microscopy tracks interactions of Bacillus subtilis with plant roots across the entire rhizosphere
Plant–environment microscopy tracks interactions of Bacillus subtilis with plant roots across the entire rhizosphere
Defective cytokinin signaling reprograms lipid and flavonoid gene-to-metabolite networks to mitigate high salinity in Arabidopsis
Defective cytokinin signaling reprograms lipid and flavonoid gene-to-metabolite networks to mitigate high salinity in Arabidopsis
Long-term breeding progress of yield, yield-related, and disease resistance traits in five cereal crops of German variety trials
Long-term breeding progress of yield, yield-related, and disease resistance traits in five cereal crops of German variety trials
A genome-wide association study reveals that the cytochrome b5 involved in seed reserve mobilization during seed germination in rice
A genome-wide association study reveals that the cytochrome b5 involved in seed reserve mobilization during seed germination in rice
Programmed genome editing by a miniature CRISPR-Cas12f nuclease
Programmed genome editing by a miniature CRISPR-Cas12f nuclease
Fine mapping and validation of a major QTL for grain weight on chromosome 5B in bread wheat
Fine mapping and validation of a major QTL for grain weight on chromosome 5B in bread wheat
Identification and fine genetic mapping of the golden pod gene (pv-ye) from the snap bean (Phaseolus vulgaris L.)
Identification and fine genetic mapping of the golden pod gene (pv-ye) from the snap bean (Phaseolus vulgaris L.)
De novo germline mutation in the dual specificity phosphatase 10 gene accelerates autoimmune diabetes
De novo germline mutation in the dual specificity phosphatase 10 gene accelerates autoimmune diabetes
Plant height heterosis is quantitatively associated with expression levels of plastid ribosomal proteins
Plant height heterosis is quantitatively associated with expression levels of plastid ribosomal proteins
Predicting moisture content during maize nixtamalization using machine learning with NIR spectroscopy
Predicting moisture content during maize nixtamalization using machine learning with NIR spectroscopy
Gene tagging via CRISPR-mediated homology-directed repair in cassava
Gene tagging via CRISPR-mediated homology-directed repair in cassava

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