Multiplex CRISPR-Cas9 Reduces Lignin Deposition and Improves Forage Quality of Alfalfa

Researchers from Oklahoma State University and partners employed a multiplex CRISPR-Cas9 mediated gene editing system to reduce shoot lignin content and improve the digestibility and nutritional values of alfalfa. The paper is published in Frontiers in Plant Science.

The forage quality of alfalfa is significantly affected by lignin disposition in cell walls during the advanced maturity stages of the plant. Previous studies have shown to improve the forage quality and digestibility of alfalfa by targeting the RNA. Hence, the researchers targeted the MsC3H gene to reduce lignin content and improve forage quality.

The results of the study show homozygous mutation of MsC3H significantly reduced the lignin content and modified the lignin composition in stems. These lines also had significantly lower levels of acid and neutral detergent fiber and higher levels of total digestible nutrients. The findings of the study accelerate the current efforts to improve alfalfa without sacrificing plant growth and biomass field.

For more information, read the article from Frontiers in Plant Science.

See https://www.isaaa.org/kc/cropbiotechupdate/ged/article/default.asp?ID=20674